2) Add in your protocol in Method Editor the Fluorescence Polarization strip. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. applications, including dual luciferase and ATP assays. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. i3. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. 価格、見積もり、お近くの販売店を. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. Turbidity measurements were performed with a Spectra Max® i3x microplate reader to study the effect of oxidation on the kinetics of sFN formation. It allows you to explore cellular pathways and protein activation and expression in one system. , 1997). SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. Explore a wealth of applications with the future-ready SpectraMax i3x. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. See SpectraMax® Microplate Reader User Manual Download Page. These robust readers have been placed in labs from Antarctica to the. Fluorescence Microplate Reader Comparison Chart. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. The SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. 1. Learn more about its features, specifications, and applications in this user guide. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Increased fluorescence performance. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Reaction catalyzed by the firefly luciferase. Nuclear Receptor. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. We would like to show you a description here but the site won’t allow us. Find regional support. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. ValitaTiter Plus settingsThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. The fluorescence quantum yield (QY) of the Pdots was measured by a UV−NIR absolute PL QY. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. The information below applies to a standard 96 well plate, set to a linear shake mode. SeeSpectraMax MiniMaxImagingCytometeronpage85. The difference in absorbance observed in the presence and absence of SOD was considered to be the amount of superoxide produced, and results. Patented user-exchangeable cartridge design expands the system's detection capability making it. SpectraDrop Abs Protein Quant (i3-i3x)Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax i2X is used for cell migration, cell. 08, 0. ) deep, preferably with access on all sides. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. It allows you to explore cellular pathways and protein activation and expression in one system. The assay can accurately test the toxicology of compounds as shown in Figure 1. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. The LDH activity in the cultural media was measured based on the reaction between LDH and lactic acid, which led to the generation of pyruvic acid, and pyruvic acid could become brown in alkaline environments with 2,4. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The device can be upgraded to include capabilities for Western blotting, imaging and injection. china@moldev. 1. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. The chamber. 12911151) 0. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. i3x. The SpectraMax ® i3x reader provides the. The SpectraMax Injector Cartridge enables precise reagent delivery and adjustment of delay and integration times that are required to obtain optimal sensitivity for this assay. D 1 1. Category 2 and 3 microplate readers use gradient heating as well. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). HTRF is a proprietary time-resolved fluoresce nce technology that overcomesThe SpectraMax i3x reader features a cooled photomultiplier tube (PMT) for low background noise in luminescence. The cell imaging was captured on Zeiss LSM 980 confocal microscopy. Accordingly, we calculated the Laurdan generalized polarization (GP) using the formula GP = (I 435 − I 490)/(I 435 +. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. The Molecular Devices SpectraMax i3x Microplate Reader is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Prime function 20 amol. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. 5 decades, with sensitive detection of cells. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Find regional support. Prime function 20 amol. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA) using the setup of excitation and emission provided in the SoftMax Pro 7. Molecular Devices are known for both the reliability and the wide uses of their products. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . Increased fluorescence performance. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. flThe SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. edu) to be added as a trained user in iLab. The treatments were. When the user applies a set temperature of 37°C, the top is heated to 37. Other services inlcude but are not limited to anti-drug activity (ADA), PK/PD assays as well as antibody screenings. Spectranet customer portal The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Ferguson, Ph. Average 98 stars, based on 1 article reviews. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. The absorbance at 450 nm was read with a Spectra Max i3X microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA), and the data at 30–40 min post-treatment were analyzed. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 3: The cuvette chamber. 312 gain calibration failed. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. ZERO BIAS - scores, article reviews, protocol conditions and more. 0. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Price from $9. SpectraMax i3x and Paradigm readers require the FP-FLUO. SpectraMax i3x 一套系统即可以帮助你通过科学手段解开细胞通路的奥秘，也可以检测并分析出 样品中蛋白质的活性和表达情况。SpectraMax i3x 检测系统可以根据未来实验需求，无需售后工 程师协助即可加入全新检测功能模块，真正做到一次投入终身受益。1) Select the correct plate and the wells that you want to measure. Multimode Reader Comparison Chart. Our patent-pending StainFree Cell Detection Algorithm enables cell counting and confluency measurements. , 2021). 2. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. The dual auto injector module. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. The chamber. Also refer to this Multimode Reader Comparison Chart. , 2011). g. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 4. We would like to show you a description here but the site won’t allow us. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. Find regional support. Description 1. Donor emission at 616 nm is used as an internal reference, while acceptor emission at 665 nm. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Download PDF. Spectramax I3x, supplied by Danaher Inc, used in various techniques. The CellTiter-Glo assay from Promega uses luciferase. The SpectraMax i3x reader provides theAbsorption was measured at 490 nm by utilizing a Spectra max i3x Multimode microplate reader (Molecular Devices). Carbonyl assay The plates for carbonyl detection were prepared as described. Videos & Webinars. As shown in the results above, the DELFIA cell proliferation assay combined with the SpectraMax i3 reader gives researchers a very sensitive test for measuring cell proliferation. 2. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyWe would like to show you a description here but the site won’t allow us. 3 In this application note we demonstrate a linear dynamic range of 6 decades for recombinant firefly and Renilla luciferases, as well as linear detection of luciferase-transfected. 304 signal level saturation. Equipped with transmitted light (TL), green (Ex/Em: 460/541) and red (625/713) fluorescence channels, the MiniMax cytometer with SoftMax® Pro Software can be used to image and. 1 mg/mL density of resazurin, protected of the light, incubated for 1 h at room temperature. 1 1 10 100 e e e e e e eThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. 99（图一）。After incubation at room temperature for one hour in the dark, we measured the fluorescence intensity using a SpectraMax i3x (Molecular Devices, CA, USA), with excitation at 350 nm and dual emissions at 435 nm and 490 nm. The SpectraMax i3 also allows the user to identify antibodies during Elisa Assays. The turbidity. Description 1. Figure 4. Microplate Reader. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. Prepare your i3/i3x instrument for shipment. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Manufacturers try to design their instruments so they can be. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataBased on the SpectraMax i3x system, ACROBiosystems provides biomedical research and development services for customers seeking quantification and detection of antibodies-antigen interaction. About the reader setup recommendations The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Find regional support. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. SpectraMax i3x. , Fisher Scienti fi c, cat. 58% for the detection module and 2. Get a front row view of your cells. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 04, 0. ATP standard curves read on SpectraMax microplate readers. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. Promega. Bioz Stars score: 98/100, based on 1 PubMed citations. Features. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. The assay window spanned four orders of magnitude. Time-. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. However, no piece of equipment is immune to breaking down. SoftMax Pro. In addition, Image J was used to quantify the. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The dynamic range of the assay is about 3. Prepare your i3/i3x instrument for shipment. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. pathways and protein activation and expression in one system. See5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideMolecular Interactions. 8 Antibodies and. HTRF IP standard curve. ZERO BIAS - scores, article reviews, protocol conditions and more. The MPO assay was conducted according to the. All test measurements and calculations are handlediD5. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . Home > Search Results > Danaher Inc > spectramax i3x. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Promega. 2 (Molecular Devices, San Jose, CA, USA). Analysis of HTRF assays involves Cisbio’s patented ratiometric reduction method based on the two emission wavelengths detected. Methods. Note: Additional readers with similar performance are listed. 313 reference gain check fail. These concentrations are then used to perform western blots. This is not due to a temperature-dependent alteration in cellular autofluorescence,. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. 314 low lamp level warning. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. 7. Sunnyvale, CA, USA) with excitation at 475 nm. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Capture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. each new assay. Explore a wealth of applications with the future-ready SpectraMax i3x. no. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreSimilar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Controls containing all reaction components with either DI . NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. Published Dec 16, 2020 | Updated Dec 16, 2020. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. To learn more about the device, please watch this short video. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. 303 unable to cal dark current. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength and superior sensitivity across the full. Fourier transform infrared (FT-IR) spectra of cCQD were obtained on a. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. Remove the transport bracket and store the retaining screws in the bag that held the 3. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Figure 2-23: Bottle holder To install the bottle holder: 1. at 540 nm using a Spectra Max i3x microplate reader (Molecular devices, San Jose, CA). D 1 1. Reporter Assay. It allows you to explore cellular . pathways and protein activation and expression in one system. 0. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Pick the best speed, sensitivity, and price for your needs. ) deep, preferably with access on all sides. 7 mm for all shake intensities. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. Key features. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. Immunofluorescence StainingAfter cooled down to room temperature, the concentrations of the solution were measured by microplate reader (Spectra Max i3x) at 490 nm (Kagi and Schaffer, 1988). SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Order Now. X-ray powder diffractometer (XRD) with Cu- Ka radiation (Bruker AXS D8) was used to identify the crystalline phase of as synthesized C-AuNPs. 001 0. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. 0 mm hex key. spectramax i3x (Danaher Inc)The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. Download PDF. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Multimode Readers Comparison Chart. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Examples: “Press carefully to engage,” “ Do not. SpectraMax® i3x Multi-Mode Microplate Reader with MiniMax™ imaging cytometer Integrated multi-mode microplate reader and imaging system • Ideal for cell counting, label-free imaging, and cell proliferation • Western blot, imaging, and injectors on one configurable microplate reader • Stain free, automated cell countingusing the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. The calculated LOQ values were 0. Figure 1. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Animal imaging assay was performed on a IVIS R Lumina Series III small animal optical system. SpectraMax® i3x 多功能读板机配置 SpectraMax® MiniMax™ 300 细胞计数 仪，具有 transmitted light (TL)，绿色 (Ex / Em: 460 / 541) 和红色 (Ex / Em: 625 / 713) 荧光通道。MiniMax 细胞计数仪的 SoftMax® Pro 软件可以用来获得各种细 胞的图像并进行分析。本说. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the datathe SpectraMax i3x Multi-Mode Microplate Reader with SpectraMax Injector Cartridge Introduction Reporter gene assays are used to study the expression of eukaryotic genes. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. HTRF IP standard curve. See SpectraMax® Microplate Reader User Manual Download Page. Features. . 3. 5. SpectraMax i3x Multi-Mode Detection Platform User Guide. 6% for the on-board optics (monochromators). The assay window spanned four orders of magnitude. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. We would like to show you a description here but the site won’t allow us. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. com The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™. 2 only) SpectraMax M-series Microplate readers. Patented user-exchangeable cartridge design expands the system's detection capability making it. 6 in. i3x. 6% for the on-board optics (monochromators). The FRET ratio is defined as the relative fluorescence intensity of an acceptor normalized to the total fluorescence intensity. 0´ 104 4. 314 low lamp level warning. com. (SpectraMax i3/i3x) CatchPoint SimpleStep ELISA (SpectraMax Paradigm) SpectraDrop. 00800 665 32860. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. As. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. Downloads. Table 1. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Microplate Readers and SoftMax® Pro Software. The SPECTRAmax 340PC can mea-Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Plate chamber design of the SpectraMax category 1 microplate readers. Get Pricing. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. 1) Select the correct plate and the wells that you want to measure. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. 0. Analysis. Data Analysis. GFP expression imaged on the cytometer. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. ZERO BIAS - scores, article reviews, protocol conditions and more. The plate was shaken and incubated in the dark for 10 minutes. 4. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologySpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev.